DNA BARCODING OF MOSQUITOES FROM THE PANTANOS DE CENTLA BIOSPHERE RESERVE, SOUTHEASTERN MEXICO
Accurate identification of mosquito species is essential to support programs that involve the study of distribution and mosquito control. Numerous mosquito species are difficult to identify based only on morphological characteristics, due to the morphological similarities in different life stages...
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| 其他作者: | , , , , , , , , |
| 格式: | Artículo |
| 语言: | en_US |
| 出版: |
2021
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| 在线阅读: | https://doi.org/10.2987/21-6967 https://meridian.allenpress.com/jamca/article/37/4/198/474265/DNA-Barcoding-of-Mosquitoes-from-the-Pantanos-de |
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| 总结: | Accurate identification of mosquito species is essential to support programs that involve the study
of distribution and mosquito control. Numerous mosquito species are difficult to identify based only on
morphological characteristics, due to the morphological similarities in different life stages and large numbers of
some species that are members of morphologically similar species complexes. In the present study, the mosquitoes
collected in the Pantanos de Centla Biosphere Reserve, southeastern Mexico, were evaluated using a combination of
morphological and molecular approaches (mitochondrial cytochrome c oxidase subunit I [COI] DNA barcode). A
total of 1,576 specimens of 10 genera and 35 species, mostly adult stages, were collected. A total of 225 COI DNA
barcode sequences were analyzed; most species formed well-supported groups in the neighbor joining, maximum
likelihood, and Bayesian inference trees. The intraspecific Kimura 2-parameter (K2P) genetic distance averaged
1.52%. An intraspecific K2P distance of 6.20% was observed in Anopheles crucians s.l., while a deep split was
identified in Culex erraticus and Cx. conspirator. This study showed that COI DNA barcodes offer a reliable
approach to support mosquito species identification in Mexico. |
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